MLVA is based on differences in numbers of tandem repeats in multiple loci on the chromosome of bacteria. For each locus the number of repeats is determined using PCRs with specific primers based on the flanking regions of the tandem repeats. Therefore, MLVA is rapid, easy to perform and reproducible. Furthermore, since MLVA types are discriminated by gain and loss of discrete repeats, this method will also provide an unambiguous nomenclature of genotypes. MLVA is like MLST a portable technique and very suitable for data exchange via Internet.
Note: there are alterations in PCR conditions compared to the publication in JCM. You will find them under DNA prep. and VNTR PCR