|
Locusname |
Repeat length(bp) |
Range no. repeats |
No.of alleles |
%Conservation |
Primer sequence |
PCR program |
Estimated size (bp) |
%Agarose gel |
|
VNTR-1
|
123
|
0-8
|
8
|
95
|
F:CTGTGATTTGGAGTTAGATGG
R:CATTGTCCAGTAGAATTAGATTTG |
30 cycli
52 C |
250-1012
|
2
|
|
VNTR-2
|
279
|
1-14
|
13
|
96
|
F:GATGCTTATTTCCACTGCTTGTTG
R:GTTTTACCCTCTCTTTTAAGGTCAATG |
TD
70-60 |
724-4351
|
1
|
|
VNTR-7
|
121
|
1-7
|
7
|
98
|
F: CTATCAGTTTCAGCTATTCCATC
R: CTGGTACGAATCAAATCAAGTG |
TD
65-55 |
416-1021
|
2
|
|
VNTR-8
|
121
|
1-7
|
7
|
96
|
F: GGGGAGTGGCAAAAAATAGTGTG
R: CAGATCATCAACTATCAACCGCTG |
TD
65-55 |
237-963
|
2
|
|
VNTR-9
|
121
|
1-3
|
3
|
93
|
F:CTGCATCTAATAACAAGGACCCATG
R: ACATTCCGATTAACGCGAAATAAG |
TD
65-55 |
205-447
|
2
|
|
VNTR-10 |
121
|
0-3
|
4
|
96
|
F: CCTACAGAAAATCCAGACGG
R: TTTTTTCCATCCTCT TGAATTG |
TD
65-55 |
174-474
|
2
|