Intracellular trafficking and the proper steady state location of biomolecules is essential for normal cell functioning. To function optimal in haemostasis, platelet-specific proteins have to be correctly targeted during megakaryocyte formation. Platelet adhesion and secretion require dynamic membrane fusion events and rearrangement of cytoskeletal elements and membrane-bound proteins. Correct targeting of peptide-loaded MHC class II molecules to the cell surface in antigen presenting cells is essential for the immune response. To better understand these subcellular trafficking pathways and to optimally visualize protein and membrane dynamics, we apply high resolution imaging techniques such as real-time imaging, confocal microscopy, immuno electron microscopy, and EM-tomography.
On this project are working under supervision of Dr. H.F.G. Heijnen:
Suzanne van Dijk, technician
Hezder van Nispen tot Pannerden, Ph.D.student