For many years, investigations in the field of oxidative stress were limited by a confusing number of surrogate markers with unclear clinical relevance.In the past decade, the search for reliable markers of oxidative stress received high priority for many research groups. A reliable marker of oxidative stress status is not only important for the design of clinical trials but also for large prospective investigations to find the potential link between lipid oxidation and incidence of IHD. Recently, stable end products of arachidonic acid oxidation, F2-isoprostanes, have been emerged as the gold standard of oxidative stress in vivo if determined in urine by Liquid Chromatography and tandem mass spectrometry (LC-MSMS) approach.
We have shown that:
- The concentrations of isoprostanes in urine are associated with smoking, age and history of cardiovascular disease, but it does not predict the incidence of atherothrombosis.
- Fluorescent products of lipid peroxidation were associated with increased incidence of ischaemic heart disease.
On this project are working under the supervision of Dr. M. Roest and Dr. H.A.M. Voorbij:
Ing. A.D. Barendrecht, technician
In collaboration with:
- Dr. T. Teerlink, VUMC, Amsterdam, the Netherlands
- Dr. P. Scheffer, VUMC, Amsterdam, the Netherlands