In the Lab of Immunopathology (Unit Molecular and ImmunoPathology; MIP) all more specialized histochemical techniques are performed. These techniques are performed on about 15-20% of all tissues handed in, and are enzyme histochemistry, Immunohistochemistry (both immunofluorescence and Immunoperoxidase techniques), and electron microscopy. In situ hybridisation is performed in combination with the Lab of Molecular Pathology. Techniques are used to improve or support the histopathological diagnostics. Immunohistochemical techniques are in recent years automated.
Enzymhistochemistry is used to characterize cells in malignancies, but also to test enzyme activities in muscle diseases and in other pathological conditions. Immunopathology is mainly used to optimize the characterization of tumor cells, but also in localizing immuno-deposits and infiltrating cells in various immunological conditions. Also viral and bacterial infections in tissues can be detected by these techniques.
Electron microscopy is used especially in supporting the morphological changes observed by light microscopy and to further characterize structural changes in cells and tissues.
Lab Molecular Pathology
Experimental Pathologist
Dr. Roel de Weger
Figure 1 Lab Immunopathology 2010
Ltr John de Pinth, Kevin van der Ven, Dorine van der Brink, sabrina Elshof, Domenico Castiliego, Filipa Monteiro and Roel de Weger
Technicians ImmunoPathology
Sabrina Elshof, chief technician
Kevin van der Ven, deputy
Marije Boer (2011)
Dorine van der Brink
Domenico Castiliego
Eline van der Esch (2011)
Petra van de Kraak (in part on AtheroExpress project)
Gladys Mancipe-Bolano (BioBank)
Filipa Monteiro
Daphne Oostenrijk
Ton Peeters (2011)
John de Pinth