Amoniocentesis study

Background uitklapper, klik om te openen

RSV LRTI is a common cause of illness in young children. About 30 % of children develop a respiratory syncytial virus (RSV) lower respiratory tract infection (LRTI) in the first year, of which about 3% are then hospitalized.

There is no cure for RSV LRTI. However, there are medications that can prevent RSV LRTI.

RSV LRTI is a common cause of illness in young children. About 30 % of children develop a respiratory syncytial virus (RSV) lower respiratory tract infection (LRTI) in the first year, of which about 3% are then hospitalized.

There is no cure for RSV LRTI. However, there are medications that can prevent RSV LRTI.

RSV LRTI is a common cause of illness in young children. About 30 % of children develop a respiratory syncytial virus (RSV) lower respiratory tract infection (LRTI) in the first year, of which about 3% are then hospitalized.

There is no cure for RSV LRTI. However, there are medications that can prevent RSV LRTI.

RSV LRTI is a common cause of illness in young children. About 30 % of children develop a respiratory syncytial virus (RSV) lower respiratory tract infection (LRTI) in the first year, of which about 3% are then hospitalized.

There is no cure for RSV LRTI. However, there are medications that can prevent RSV LRTI.

It is therefore important to identify children with a high probability of (severe) RSV lower respiratory tract infections at an early stage and ensure patient preventive treatment protocols are followed. Known risk factors include premature birth, children with congenital heart defects and children with Down syndrome. However, 90% of the RSV LRTI occurs in children with no known risk factors. This study investigates the influence of inflammation around the birth (amniotic fluid, placenta and umbilical cord blood) on the development of RSV LRTI.

Intra -uterine infection is associated with an increased risk of premature birth. Intrauterine infection may also play a role in the disruption of a normal delivery. However, little is known about inflammatory markers in amniotic fluid and placenta in normal pregnancies and births. During pregnancy, the lungs of the fetus are filled with amniotic fluid. Pregnancy and early neonatal period are crucial for lung maturation. This study of amniotic fluid examines the role of intrauterine infection during childbirth and the onset of RSV LRTI in later life.

The immune system of newborn children differs from the immune system of adults. On the one hand newborns have less contact with pathogens. Yet, there are large differences in the innate (non-specific) immune system. Toll-like receptors are an important part of the innate immune system. Many laboratory studies suggest a link between Toll-like receptors (RSV) virus infections. In this study, we investigate the role of receptor responses in the pathogenesis of RSV respiratory infections.

Intra -uterine infection is associated with an increased risk of premature birth. Intrauterine infection may also play a role in the disruption of a normal delivery. However, little is known about inflammatory markers in amniotic fluid and placenta in normal pregnancies and births. During pregnancy, the lungs of the fetus are filled with amniotic fluid. Pregnancy and early neonatal period are crucial for lung maturation. This study of amniotic fluid examines the role of intrauterine infection during childbirth and the onset of RSV LRTI in later life.

The immune system of newborn children differs from the immune system of adults. On the one hand newborns have less contact with pathogens. Yet, there are large differences in the innate (non-specific) immune system. Toll-like receptors are an important part of the innate immune system. Many laboratory studies suggest a link between Toll-like receptors (RSV) virus infections. In this study, we investigate the role of receptor responses in the pathogenesis of RSV respiratory infections.

Aim of the study uitklapper, klik om te openen

In addition, a number of sub targets:

To investigate whether inflammatory factors surrounding the birth can be associated with the method of delivery.
To investigate whether inflammatory factors around birth can be associated with lung function at the age of one month.
Examine if lung function at the age of one month associated with the risk of RSV LRTI.

In addition, a number of sub targets:

To investigate whether inflammatory factors surrounding the birth can be associated with the method of delivery.
To investigate whether inflammatory factors around birth can be associated with lung function at the age of one month.
Examine if lung function at the age of one month associated with the risk of RSV LRTI.

In addition, a number of sub targets:

To investigate whether inflammatory factors surrounding the birth can be associated with the method of delivery.
To investigate whether inflammatory factors around birth can be associated with lung function at the age of one month.
Examine if lung function at the age of one month associated with the risk of RSV LRTI.

In addition, a number of sub targets:

To investigate whether inflammatory factors surrounding the birth can be associated with the method of delivery.
To investigate whether inflammatory factors around birth can be associated with lung function at the age of one month.
Examine if lung function at the age of one month associated with the risk of RSV LRTI.

This study has two primary goals:

We shall investigate whether there is a link between inflammation during pregnancy (in amniotic fluid and placenta), and the risk of RSV LRTI in the first year of life.
Investigate whether there is a connection between the function of the innate immune system at birth and risk of RSV LRTI in the first year of life.

This study has two primary goals:

We shall investigate whether there is a link between inflammation during pregnancy (in amniotic fluid and placenta), and the risk of RSV LRTI in the first year of life.
Investigate whether there is a connection between the function of the innate immune system at birth and risk of RSV LRTI in the first year of life.

This study has two primary goals:

We shall investigate whether there is a link between inflammation during pregnancy (in amniotic fluid and placenta), and the risk of RSV LRTI in the first year of life.
Investigate whether there is a connection between the function of the innate immune system at birth and risk of RSV LRTI in the first year of life.

This study has two primary goals:

We shall investigate whether there is a link between inflammation during pregnancy (in amniotic fluid and placenta), and the risk of RSV LRTI in the first year of life.
Investigate whether there is a connection between the function of the innate immune system at birth and risk of RSV LRTI in the first year of life.

Participant Groups/ Population uitklapper, klik om te openen

In this study, we looked at healthy children, born in the Wilhelmina Children's Hospital (Utrecht) and the Diakonessenhuis (Utrecht). Participants are divided into subgroups based on:
                       Gestational age (<37 wk or ≥ 37 wk)
                        Method of delivery (spontaneous delivery or cesarean)
                        Duration of ruptured membranes (< 24 or ≥ 24 hours)

Children with congenital heart or lung disease, other serious illnesses and children of parents with insufficient command of the Dutch language cannot participate in this study.

Study components uitklapper, klik om te openen

Amniotic fluid: For participants in the study at birth captured a small amount of amniotic fluid. Written permission from parents is investigated on inflammatory cells and proteins. In addition, the placenta is examined for signs of inflammation.

Blood: Umbilical cord blood, taken immediately after the birth, is examined for inflammatory cells and proteins. This study will be repeated in blood that is taken at the age of four weeks. We shall also conduct cord blood research into genes for hereditary predisposition to inflammation and immunity.

Pulmonary function testing: At the age of four weeks, lung function tests will take place. In this technique, breathing is measured during complete rest (sleep). This research is not burdensome upon the patient.

Cold symptom Diary: Parents of participants write all respiratory symptoms in a diary from birth to the first birthday of their child.

Nose Sample (mucus): During each cold during the first year of life, parents take a nasal mucus sample with cotton swab. This mucus is then examined by the team for the presence of cold viruses.

Questionnaire: Around the first birthday of the participant(s), parents receive a questionnaire on respiratory symptoms and overall quality of life. The doctor will also ask if there are established respiratory problems in the first year of life.

Amniotic fluid: For participants in the study at birth captured a small amount of amniotic fluid. Written permission from parents is investigated on inflammatory cells and proteins. In addition, the placenta is examined for signs of inflammation.

Blood: Umbilical cord blood, taken immediately after the birth, is examined for inflammatory cells and proteins. This study will be repeated in blood that is taken at the age of four weeks. We shall also conduct cord blood research into genes for hereditary predisposition to inflammation and immunity.

Pulmonary function testing: At the age of four weeks, lung function tests will take place. In this technique, breathing is measured during complete rest (sleep). This research is not burdensome upon the patient.

Cold symptom Diary: Parents of participants write all respiratory symptoms in a diary from birth to the first birthday of their child.

Nose Sample (mucus): During each cold during the first year of life, parents take a nasal mucus sample with cotton swab. This mucus is then examined by the team for the presence of cold viruses.

Questionnaire: Around the first birthday of the participant(s), parents receive a questionnaire on respiratory symptoms and overall quality of life. The doctor will also ask if there are established respiratory problems in the first year of life.

Outcome measured uitklapper, klik om te openen

Other study ( secondary outcomes ) : The number of episodes of wheezing in later life , measured by questionnaires at the ages of 3 and 6 years ; Development of asthma , as measured by questionnaires to parents and doctor at the age of 3 and 6 years ; The health - related quality of life ; The socio-economic impact of RSV and recurrent episodes of wheezing (e.g., cost of medication , doctor visits and hospitalizations , parental absenteeism from work).

The main outcome to be measured in this study is the prevalence of RSV LRTI. This is determined by presence and severity of RSV lower respiratory tract infections on the basis of the data from the nasal mucus examination; The number of days of wheezing during the first year of life is determined by the information tracked in the parental diary, the questionnaire at the information collected by patients general practitioner.