Back

prof. dr. R.J.L. (Rob) Willems

Full Professor

  • Department of Medical Microbiology

prof. dr. R.J.L. (Rob) Willems

Research Programs

Biography

Prof. dr. Rob Willems studied biology at the Free University in Amsterdam and acquired his PhD from the Utrecht University in 1993 on “Genetic and functional studies on Bordetella pertussis fimbriae”. After postdoc positions at the Faculty of Veterinary Medicine, Utrecht University and at the Department of Infectious Diseases of the University Hospital, Leiden he became research scientist and section head at the National Institute for Public Health and the Environment (RIVM) in 1996. In 2003 he moved from the RIVM to the University Medical Center Utrecht where he became assistant professor. In 2008 he was appointed associate professor and since December 1st 2016, he is full professor of Population Genetics of Antibiotic Resistance at the department of Medical Microbiology of the University Medical Center Utrecht. Since 1997, he has set up and been involved in various national and international studies on antibiotic resistance. From 2007-2011 he coordinated an EC FP6 project on Approaches to Control multi-resistant Enterococci (ACE) and from 2011-2015 he was principal investigator and coordinator of the EC FP7 project designated Evolution and Transmission of Antibiotic Resistance (EvoTAR). His research group aims to unravel the genetic evolution, population and functional genomics of Enterococcus faecium and Enterococcus faecalis as well as E. faecium host-pathogen interactions. His group was the first that identified structure in the E. faecium population and described that strains from hospitalized patients were genetically distinct from animal isolates and human commensal isolates. He also published the first comparative genomics paper on E. faecium using comparative genomic hybridizations as well as whole genome sequencing that revealed that E. faecium has an open genome capable for frequently incorporating new DNA in its genome. Currently, his group is also focussing on genome based epidemiology of multidrug-resistant pathogens, with a special focus reconstructing and tracking the spread of plasmids based on genome sequence data. Furthermore, his group aims to decipher the processes and molecular mechanisms that contribute to colonization resistance against high density colonization of antibiotic-resistant Enterobacteriaceae and enterococci and the consequences of intestinal outgrowth of these multidrig resistant opportunistic pathogens on pathogen-host cross-talk. Dr. Willems has authored more than 175 peer-reviewed papers and book chapters.

Side Activities

  • Chair of the Division of Molecular typing of the Royal Netherlands Society for Microbiology. Organised and lectured in courses and conferences in microbiology in Utrecht, Amsterdam, Wageningen, France, Germany, United Kingdon, Norway, Denmark, and Italy. Referee for several journals and regular referee for funding organisations such as University of Tromso (Norway),Institut Pasteur (France), Irish Research Council Laureate Awards (Ireland) and German Research Foundation, DTU (Denmark), International Centre for diarrhoeal Disease Research  (Bangladesh), Marie Curie Fellowship (EU), NWO-Top (NL), ZonMW-Veni (NL) and The Welcome Trust (UK).

Research Output (223)

Low IgA Associated With Oropharyngeal Microbiota Changes and Lung Disease in Primary Antibody Deficiency

Berbers Roos Marijn, Mohamed Hoesein Firdaus A.A., Ellerbroek Pauline M., van Montfrans Joris M., Dalm Virgil A.S.H., van Hagen P. Martin, Paganelli Fernanda L., Viveen Marco C., Rogers Malbert R.C., de Jong Pim A., Uh Hae Won, Willems Rob J.L., Leavis Helen L. 19 jun 2020, In: Frontiers in Immunology. 11 11 p.

gplas:a comprehensive tool for plasmid analysis using short-read graphs

Arredondo-Alonso Sergio, Bootsma Martin, Hein Yaïr, Rogers Malbert R.C., Corander Jukka, Willems Rob J.L., Schürch Anita C. 15 jun 2020, In: Bioinformatics (Oxford, England). 36 , p. 3874-3876 3 p.

Nonclonal Emergence of Colistin Resistance Associated with Mutations in the BasRS Two-Component System in Escherichia coli Bloodstream Isolates

Janssen Axel B., Bartholomew Toby L., Marciszewska Natalia P., Bonten Marc J.M., Willems Rob J.L., Bengoechea Jose A., van Schaik Willem 11 mrt 2020, In: mSphere. 5 13 p.

Plasmids shaped the recent emergence of the major nosocomial pathogen Enterococcus faecium

Arredondo-Alonso S., Top J., McNally A., Puranen S., Pesonen M., Pensar J., Marttinen P., Braat J. C., Rogers M. R.C., van Schaik W., Kaski S., Willems R. J.L., Corander J., Schürch A. C. 11 feb 2020, In: mBio. 11 17 p.

Extended-spectrum beta-lactamase (ESBL)-producing and non-ESBL-producing Escherichia coli isolates causing bacteremia in the Netherlands (2014 - 2016) differ in clonal distribution, antimicrobial resistance gene and virulence gene content

van Hout Denise, Verschuuren Tess D, Bruijning-Verhagen Patricia C J, Bosch Thijs, Schürch Anita C, Willems Rob J L, Bonten Marc J M, Kluytmans Jan A J W 1 jan 2020, In: PLoS ONE. 15 14 p.

In vivo acquisition of fosfomycin resistance in Escherichia coli by fosA transmission from commensal flora

Ten Doesschate Thijs, Abbott Iain J., Willems Rob J.L., Top Janetta, Rogers Malbert R.C., Bonten Marc M., Paganelli Fernanda L. 1 dec 2019, In: The Journal of antimicrobial chemotherapy. 74 , p. 3630-3632 3 p.

Low-calcium diet in mice leads to reduced gut colonization by Enterococcus faecium

Top Janetta, Hendrickx Antoni P.A., van Ampting Marleen T.J., van Limpt Kees, Knol Jan, van de Kamer Denise, Braat Johanna C., Viveen Marco, Rogers Malbert R., Kemperman Hans, Willems Rob J.L., Paganelli Fernanda L. 1 dec 2019, In: MicrobiologyOpen. 8 6 p.

CRISPR-Cas9-mediated genome editing in vancomycin-resistant Enterococcus faecium

de Maat Vincent, Stege Paul B., Dedden Mark, Hamer Maud, van Pijkeren Jan Peter, Willems Rob J.L., van Schaik Willem 1 nov 2019, In: FEMS microbiology letters. 366

Amplified fragment length polymorphism and whole genome sequencing:A comparison of methods in the investigation of a nosocomial outbreak with vancomycin resistant enterococci

Janes Victoria A., Notermans Daan W., Spijkerman Ingrid J.B., Visser Caroline E., Jakobs Marja E., Van Houdt Robin, Willems Rob J.L., De Jong Menno D., Schultsz Constance, Matamoros Sébastien 23 sep 2019, In: Antimicrobial Resistance and Infection Control. 8

Phylogenomics of Enterococcus faecalis from wild birds:new insights into host-associated differences in core and accessory genomes of the species

León-Sampedro Ricardo, del Campo Rosa, Rodriguez-Baños Mercedes, Lanza Val F., Pozuelo María José, Francés-Cuesta Carlos, Tedim Ana P., Freitas Ana R., Novais Carla, Peixe Luísa, Willems Rob J.L., Corander Jukka, González Candelas Fernando, Baquero Fernando, Coque Teresa M. aug 2019, In: Environmental Microbiology. 21 , p. 3046-3062 17 p.

All Research Output (223)